EMD-20439

Single-particle
12.2 Å
EMD-20439
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Deposition: 07/07/2019
Map released: 08/01/2020
Last modified: 08/01/2020
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-20439
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Cryo-EM reconstruction of the immature chimeric BinJV/ZIKV-prME virion

EMD-20439

Single-particle
12.2 Å
EMD-20439
3D View Gallery
Deposition: 07/07/2019
Map released: 08/01/2020
Last modified: 08/01/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 2 mg/mL
Details: Gradient purified BinJV/ZIKV-prME
Buffer
pH: 8.0
Grid
Details: Unspecified
Support Film [1]
Material Topology Thickness
CARBON LACEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277.15 K
Instrument: FEI VITROBOT MARK II
Microscopy [1]
Microscope: FEI TECNAI F30
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 26.7 e/Å2
Image processing [1]
Final reconstruction
Resolution: 12.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Number of images used: 1075
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
RELION 3.0 -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-8508
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
CTF correction
Software [1]
Name Version Details
CTFFIND 4 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Single particle reconstruction of the immature chimeric BinJV/ZIKV-prME virion
⬡ Geometry
X Y Z
Dimensions 384 384 384
Origin 0 0 0
Spacing 384 384 384
Voxel size 2.027 Å 2.027 Å 2.027 Å
Contour list
Primary Level Source
True 0.02 AUTHOR