EMDB EMD-20435

Single particle reconstruction
3.4Å resolution

Bacterial 45SRbgA ribosomal particle class B

Map released:
Overview of EMD-20435
Source organism: Bacillus subtilis [1423]
Fitted atomic model: 6ppf
Primary publication:
RbgA Triggers Folding of the 50S Subunit Functional Sites
Seffouh A, Jain N, Jahagirdar D, Basu K, Razi A, Ni X, Guarne A, Britton RA, Ortega J
Nucleic Acids Res. - (2019)

Function and Biology Details

Sample name: 45SRbgA ribosomal assembly intermediate
Ligand: water
Nucleic acids: 23S rRNA, 5S rRNA
Proteins: 45SRbgA ribosomal assembly intermediate, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L27, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L29, 50S ribosomal protein L34

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)