EMDB EMD-20327

Single particle reconstruction
4.2Å resolution

Spastin Hexamer in complex with substrate peptide

Map released:
Last modified:
Overview of EMD-20327
Source organisms:
Fitted atomic model: 6pek
Related EM entry by publication: EMD-20805
Primary publication:
Structure of spastin bound to a glutamate-rich peptide implies a hand-over-hand mechanism of substrate translocation.
Han H, Schubert HL, McCullough J, Monroe N, Purdy MD, Yeager M, Sundquist WI, Hill CP
J.Biol.Chem. 295 435-443 (2020)
PMID: 31767681

Function and Biology Details

Sample name: Spastin Hexamer in complex with substrate peptide
Ligands: ADENOSINE-5'-DIPHOSPHATE, BERYLLIUM TRIFLUORIDE ION, MAGNESIUM ION
Proteins: Spastin Hexamer in complex with substrate peptide, Spastin, substrate peptide, TYR-GLU-TYR-GLU-TYR-GLU-TYR-GLU

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)