EMDB EMD-20284

Single particle reconstruction
3.0Å resolution

Cryo-EM structure of Urocortin 1-bound Corticotropin-releasing factor 1 receptor in complex with Gs protein and Nb35

Map released:
Last modified:
Overview of EMD-20284
Source organisms:
Fitted atomic model: 6pb0
Related EM entry by publication: EMD-20285
Primary publication:
Molecular Basis for Hormone Recognition and Activation of Corticotropin-Releasing Factor Receptors.
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol.Cell 77 669- (2020)
PMID: 32004470

Function and Biology Details

Sample name: Urocortin1-bound CRF1R in complex Gs and Nb35
Ligands: CHOLESTEROL, PALMITIC ACID
Proteins: Urocortin1-bound CRF1R in complex Gs and Nb35, Corticotropin-releasing factor receptor 1, Urocortin, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Nanobody 35

Experimental Information Details

Resolution: 3.0Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 BASE (4k x 4k)