EMDB EMD-20240

Single particle reconstruction
3.2Å resolution

Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor

Map released:
Overview of EMD-20240
Source organism: Saccharomyces cerevisiae W303 [580240]
Fitted atomic model: 6p2r
Primary publication:
Structure of the eukaryotic protein O-mannosyltransferase Pmt1-Pmt2 complex
Bai L, Li H
Nat.Struct.Mol.Biol. - (2019)

Function and Biology Details

Sample name: Protein O-mannosyl transferase complex
Ligands: N-ACETYL-D-GLUCOSAMINE, 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE, (3R)-3,31-dimethyl-7,11,15,19,23,27-hexamethylidenedotriacont-31-en-1-yl dihydrogen phosphate
Proteins: Protein O-mannosyl transferase complex, Dolichyl-phosphate-mannose--protein mannosyltransferase 1, Dolichyl-phosphate-mannose--protein mannosyltransferase 2

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: Coot, UCSF Chimera
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)