EMDB EMD-20226

Single particle reconstruction
3.2Å resolution

Spastin hexamer in complex with substrate

Map released:
Overview of EMD-20226
Source organism: Drosophila melanogaster [7227]
Fitted atomic model: 6p07
Primary publication:
An allosteric network in spastin couples multiple activities required for microtubule severing
Sandate CR, Szyk A, Zehr E, Lander GC, Roll-Mecak A
Nature Structural and Molecular Biology - (2019)

Function and Biology Details

Sample name: complex of spastin homohexamer bound to polyglutmate peptide
Ligands: ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE
Proteins: complex of spastin homohexamer bound to polyglutmate peptide, Spastin, polyglutamate peptide

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TALOS ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)