EMDB EMD-20220

Single particle reconstruction
3.5Å resolution

CRYO-EM STRUCTURE OF PHOSPHORYLATED AP-2 (mu E302K) BOUND TO NECAP IN THE PRESENCE OF SS DNA

Map released:
Overview of EMD-20220
Source organisms:
Fitted atomic model: 6oxl
Related EM entry by publication: EMD-20215
Primary publication:
A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex.
Partlow EA, Baker RW, Beacham GM, Chappie JS, Leschziner AE, Hollopeter G
Elife 8 - (2019)
PMID: 31464684

Function and Biology Details

Sample name: Phosphorylated AP2-NECAP (mu E302K) co-complex in the presence of ss DNA
Proteins: Phosphorylated AP2-NECAP (mu E302K) co-complex in the presence of ss DNA, AP-2 complex subunit alpha-2, AP-2 complex subunit beta, AP-2 complex subunit mu, AP-2 complex subunit sigma, Adaptin ear-binding coat-associated protein 2, Unknown region of Adaptin ear-binding coat-associated protein 2 Ex-domain

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TALOS ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)