EMDB EMD-20215

Single particle reconstruction
3.2Å resolution

CRYO-EM STRUCTURE OF PHOSPHORYLATED AP-2 CORE BOUND TO NECAP

Map released:
Overview of EMD-20215
Source organisms:
Fitted atomic model: 6owo
Related EM entry by publication: EMD-20220
Primary publication:
A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex.
Partlow EA, Baker RW, Beacham GM, Chappie JS, Leschziner AE, Hollopeter G
Elife 8 - (2019)
PMID: 31464684

Function and Biology Details

Sample name: PHOSPHORYLATED AP2-NECAP CO-COMPLEX
Proteins: PHOSPHORYLATED AP2-NECAP CO-COMPLEX, AP-2 complex subunit alpha-2, AP-2 complex subunit beta, AP-2 complex subunit mu, AP-2 complex subunit sigma, Adaptin ear-binding coat-associated protein 2

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TALOS ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)