EMDB EMD-20191

Single particle reconstruction
3.5Å resolution

Cryo-EM structure of vaccine-elicited antibody 0PV-b.01 in complex with HIV-1 Env BG505 DS-SOSIP and antibodies VRC03 and PGT122

Map released:
Overview of EMD-20191
Source organisms:
Fitted atomic model: 6ot1
Related EM entry by publication: EMD-20189, EMD-9359
Primary publication:
Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization.
Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, Cheng C, Dingens AS, Gorman J, Sastry M, Shen CH, Zhang B, Zhou T, Chuang GY, Chao CW, Gu Y, Jafari AJ, Louder MK, O'Dell S, Rowshan AP, Viox EG, Wang Y, Choi CW, Corcoran MM, Corrigan AR, Dandey VP, Eng ET, Geng H, Foulds KE, Guo Y, Kwon YD, Lin B, Liu K, Mason RD, Nason MC, Ohr TY, Ou L, Rawi R, Sarfo EK, Schon A, Todd JP, Wang S, Wei H, Wu W, Mullikin JC, Bailer RT, Doria-Rose NA, Karlsson Hedestam GB, Scorpio DG, Overbaugh J, Bloom JD, Carragher B, Potter CS, Shapiro L, Kwong PD, Mascola JR
Cell 178 567-584.e19 (2019)
PMID: 31348886

Function and Biology Details

Sample name: HIV-1 Env BG505 DS-SOSIP and antibodies 0PV-b.01, VRC03 and PGT122
Ligands: N-ACETYL-D-GLUCOSAMINE, BETA-D-MANNOSE, ALPHA-D-MANNOSE
Proteins: HIV-1 Env BG505 DS-SOSIP and antibodies 0PV-b.01, VRC03 and PGT122, Envelope glycoprotein gp120, Envelope glycoprotein gp41, VRC03, PGT122, 0PV-b.01, Envelope glycoprotein gp41, BG505 gp120, 0PV-b.01 heavy, 0PV-b.01 light, PGT122 heavy, PGT122 light, VRC03 heavy, VRC03 light

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C3
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)