EMDB EMD-20190

Single particle reconstruction
3.84Å resolution

Structure of human Smoothened-Gi complex

Map released:
Last modified:
Overview of EMD-20190
Source organisms:
Fitted atomic model: 6ot0
Primary publication:
Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric Gi.
Qi X, Liu H, Thompson B, McDonald J, Zhang C, Li X
Nature - (2019)
PMID: 31168089

Function and Biology Details

Sample name: Smoothened-Gi-Fab complex
Ligand: 17-[3-(3,3-DIMETHYL-OXIRANYL)-1-METHYL-PROPYL]-10,13-DIMETHYL-2,3,4,7,8,9,10,11,12,13,14,15,16,17-TETRADECAHYDRO-1H-CYCLOPENTA[A]PHENANTHREN-3-OL
Proteins: Smoothened-Gi-Fab complex, Smoothened homolog, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Fab light chain, Fab heavy chain, Smoothened homolog, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Fab light chain, Fab heavy chain

Experimental Information Details

Resolution: 3.84Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)