EMDB EMD-20175

Single particle reconstruction
4.05Å resolution

Modified BG505 SOSIP-based immunogen RC1 in complex with the elicited V3-glycan patch bNAb 10-1074

Map released:
Overview of EMD-20175
Source organisms:
Fitted atomic model: 6orn
Related EM entries by publication: EMD-20176, EMD-20177, EMD-20178
Primary publication:
Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques.
Escolano A, Gristick HB, Abernathy ME, Merkenschlager J, Gautam R, Oliveira TY, Pai J, West Jr AP, Barnes CO, Cohen AA, Wang H, Golijanin J, Yost D, Keeffe JR, Wang Z, Zhao P, Yao KH, Bauer J, Nogueira L, Gao H, Voll AV, Montefiori DC, Seaman MS, Gazumyan A, Silva M, McGuire AT, Stamatatos L, Irvine DJ, Wells L, Martin MA, Bjorkman PJ, Nussenzweig MC
Nature - (2019)
PMID: 31142836

Function and Biology Details

Sample name: Complex of RC1 variant of BG505 SOSIP.664 trimer with three 10-1074 Fabs and three CD4bs Fabs
Ligands: N-ACETYL-D-GLUCOSAMINE, BETA-D-MANNOSE, ALPHA-D-MANNOSE
Proteins: Complex of RC1 variant of BG505 SOSIP.664 trimer with three 10-1074 Fabs and three CD4bs Fabs, RC1 variant of BG505 SOSIP.664 trimer, 10-1074 Fab, RC1 variant of HIV-1 Env glycoprotein gp41, RC1 variant of HIV-1 Env glycoprotein gp120, 10-1074 antibody Fab heavy chain, 10-1074 antibody Fab light chain

Experimental Information Details

Resolution: 4.05Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C3
Reconstruction software: cryoSPARC
Microscope: FEI TALOS ARCTICA
Detector: FEI FALCON III (4k x 4k)