EMDB EMD-20149

Single particle reconstruction
3.7Å resolution

Cdc48 Hexamer in a complex with substrate and Shp1(Ubx Domain)

Map released:
Overview of EMD-20149
Source organism: Baker's yeast [4932]
Fitted atomic model: 6opc
Related EM entries by publication: EMD-20124, EMD-20136, EMD-20137, EMD-20138
Primary publication:
Structure of the Cdc48 segregase in the act of unfolding an authentic substrate.
Cooney I, Han H, Stewart MG, Carson RH, Hansen DT, Iwasa JH, Price JC, Hill CP, Shen PS
Science - (2019)
PMID: 31249134

Function and Biology Details

Sample name: Cdc48-Substrate Complex
Ligands: ADENOSINE-5'-DIPHOSPHATE, BERYLLIUM TRIFLUORIDE ION, MAGNESIUM ION
Proteins: Cdc48-Substrate Complex, Cell division control protein 48, Substrate bound to the central pore of the Cdc48 hexamer, UBX domain-containing protein 1

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TALOS ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)