EMDB EMD-20133

Single particle reconstruction
3.0Å resolution

Lon Protease from Yersinia pestis with Y2853 substrate

Map released:
Last modified:
Overview of EMD-20133
Source organism: Yersinia pestis [632]
Fitted atomic model: 6on2
Related EM entry by publication: EMD-21009
Primary publication:
Structural basis for distinct operational modes and protease activation in AAA+ protease Lon.
Shin M, Puchades C, Asmita A, Puri N, Adjei E, Wiseman RL, Karzai AW, Lander GC
Sci Adv 6 eaba8404-eaba8404 (2020)
PMID: 32490208

Function and Biology Details

Sample name: Lon protease bound to Y2853 substrate
Ligands: ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE
Proteins: Lon protease bound to Y2853 substrate, ATP-dependent protease La, Bound Y2853 Substrate

Experimental Information Details

Resolution: 3.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TALOS ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)