EMDB EMD-20059

Single particle reconstruction
3.4Å resolution

In situ structure of Rotavirus RNA-dependent RNA polymerase at duplex-open state

Map released:
Last modified:
Overview of EMD-20059
Source organism: Rotavirus A [28875]
Fitted atomic model: 6ogy
Related EM entry by publication: EMD-20060
Primary publication:
In situ structures of rotavirus polymerase in action and mechanism of mRNA transcription and release.
Ding K, Celma CC, Zhang X, Chang T, Shen W, Atanasov I, Roy P, Zhou ZH
Nat Commun 10 2216-2216 (2019)
PMID: 31101900

Function and Biology Details

Sample name: Rotavirus A
Nucleic acids: DNA/RNA (5'-D(*(GTG))-R(P*GP*C)-3'), RNA (5'-R(P*AP*GP*CP*C)-3')
Proteins: RNA-dependent RNA polymerase of rotavirus A, Inner capsid protein VP2
Virus: Rotavirus A

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)