EMDB EMD-20051

Single particle reconstruction
4.1Å resolution

CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, NTD-trimer

Map released:
Last modified:
Overview of EMD-20051
Source organisms:
Fitted atomic model: 6og3
Related EM entries by publication: EMD-20004, EMD-20005, EMD-20049, EMD-20050
Primary publication:
Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase.
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun 10 2393-2393 (2019)
PMID: 31160557

Function and Biology Details

Sample name: hyperactive ClpB mutant K476C bound to casein
Proteins: hyperactive ClpB mutant K476C bound to casein, hyperactive ClpB mutant K476C_NTD trimer, alpha-s1-casein, Hyperactive disaggregase ClpB, Alpha S1-casein

Experimental Information Details

Resolution: 4.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)