EMDB EMD-1970

Single particle reconstruction
14.0Å resolution

Negative stained image reconstruction of HIV spike protein in complex with PGT128 Fab at 14 Angstrom resolution

Map released:
Overview of EMD-1970
Source organisms:
Primary publication:
A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield.
Pejchal R, Doores KJ, Walker LM, Khayat R, Huang PS, Wang SK, Stanfield RL, Julien JP, Ramos A, Crispin M, Depetris R, Katpally U, Marozsan A, Cupo A, Maloveste S, Liu Y, McBride R, Ito Y, Sanders RW, Ogohara C, Paulson JC, Feizi T, Scanlan CN, Wong CH, Moore JP, Olson WC, Ward AB, Poignard P, Schief WR, Burton DR, Wilson IA
SCIENCE 334 1097-1103 (2011)
PMID: 21998254

Function and Biology Details

Sample name: HIV spike protein 664G construct in complex with Fab fragment of PGT128 monoclonal antibody
Proteins: Human immunodeficiency virus envelope protein, PGT128 monoclonal antibody fragment

Experimental Information Details

Resolution: 14.0Å
Resolution method: FSC 0.5
Applied symmetry: C3
Reconstruction software: Sparx
Microscope: FEI TECNAI F20
Detector: GENERIC GATAN (4k x 4k)