EMD-18194
Cryo-electron tomogram of GEM2-labelled Mito-EGFP in HeLa cells
EMD-18194
Tomography13.7 Å
Deposition: 14/08/2023Map released: 30/08/2023
Last modified: 20/12/2023
Sample Organism:
Synechococcus elongatus PCC 7942 = FACHB-805
Sample: Cryo-focused ion beam lamella of a HeLa cell expressing an engineered genetically encoded multimeric tag GEM2 coupled to stably expressed Mito-EGFP upon treatment with rapalog
Deposition Authors: Fung HKH
,
Hayashi Y ,
Salo VT ,
Babenko A ,
Zagoriy I ,
Brunner A ,
Ellenberg J ,
Mueller CW ,
Cuylen-Haering S ,
Mahamid J
Sample: Cryo-focused ion beam lamella of a HeLa cell expressing an engineered genetically encoded multimeric tag GEM2 coupled to stably expressed Mito-EGFP upon treatment with rapalog
Deposition Authors: Fung HKH
,
Hayashi Y ,
Salo VT ,
Babenko A ,
Zagoriy I ,
Brunner A ,
Ellenberg J ,
Mueller CW ,
Cuylen-Haering S ,
Mahamid J
Genetically encoded multimeric tags for subcellular protein localization in cryo-EM.
Fung HKH ,
Hayashi Y ,
Salo VT ,
Babenko A ,
Zagoriy I ,
Brunner A ,
Ellenberg J ,
Muller CW ,
Cuylen-Haering S ,
Mahamid J
(2023) Nat Methods , 20 , 1900 - 1908
,
Hayashi Y ,
Salo VT ,
Babenko A ,
Zagoriy I ,
Brunner A ,
Ellenberg J ,
Muller CW ,
Cuylen-Haering S ,
Mahamid J
(2023) Nat Methods , 20 , 1900 - 1908
Grant Support:
- German Research Foundation (DFG): 419120233 (Germany)
- EIPOD fellowship under Marie Sklodowska-Curie Actions COFUND: 664726 (Germany)
- H2020 Marie Curie Actions of the European Commission: 101028297 (European Union)
- Human Frontier Science Program (HFSP): CDA00045/2019 (France)
- Other private: Biomedicum Helsinki Foundation (Finland)
- Other private: Orion Foundation (Finland)