EMD-14497

Single-particle
3.49 Å
EMD-14497
3D View Gallery
Deposition: 03/03/2022
Map released: 31/08/2022
Last modified: 09/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-14497

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-7z4h summary report (pdf.gz) Map-7z4h FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

SpCas9 bound to 14-nucleotide complementary DNA substrate

EMD-14497

Single-particle
3.49 Å
EMD-14497
3D View Gallery
Deposition: 03/03/2022
Map released: 31/08/2022
Last modified: 09/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes
Sample: Ternary complex of SpCas9-sgRNA bound to a 14-nucleotide complementary DNA substrate
Fitted models: 7z4h

Deposition Authors: Pacesa M , Jinek M
R-loop formation and conformational activation mechanisms of Cas9.
Pacesa M , Loeff L , Querques I , Muckenfuss LM , Sawicka M , Jinek M
(2022) Nature , 609 , 191 - 196
PUBMED: 36002571
DOI: doi:10.1038/s41586-022-05114-0
ISSN: 1476-4687
ASTM: NATUAS
Grant Support: