EMD-13406
AMP-PNP bound nanodisc reconstituted MsbA with nanobodies, spin-labeled at position T68C
EMD-13406
Single-particle2.8 Å
Deposition: 16/08/2021Map released: 24/08/2022
Last modified: 02/07/2025
Sample Organism:
Escherichia coli (strain K12),
Vicugna pacos
Sample: AMP-PNP bound E. coli MsbA in complex with nanobody Nb_MsbA#1, labeled with Gd-DOTA at T68C
Fitted models: 7ph4
Deposition Authors: Januliene D
,
Parey K ,
Galazzo L ,
Meier G ,
Vecchis D,
Striednig B ,
Hilbi H ,
Schaefer LV,
Kuprov I ,
Bordignon E ,
Seeger MA ,
Moeller A
Sample: AMP-PNP bound E. coli MsbA in complex with nanobody Nb_MsbA#1, labeled with Gd-DOTA at T68C
Fitted models: 7ph4
Deposition Authors: Januliene D
,
Parey K ,
Galazzo L ,
Meier G ,
Vecchis D,
Striednig B ,
Hilbi H ,
Schaefer LV,
Kuprov I ,
Bordignon E ,
Seeger MA ,
Moeller A
The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells.
Galazzo L ,
Meier G ,
Januliene D ,
Parey K ,
De Vecchis D ,
Striednig B ,
Hilbi H ,
Schafer LV ,
Kuprov I ,
Moeller A,
Bordignon E ,
Seeger MA
(2022) Sci Adv , 8 , eabn6845 - eabn6845
,
Meier G ,
Januliene D ,
Parey K ,
De Vecchis D ,
Striednig B ,
Hilbi H ,
Schafer LV ,
Kuprov I ,
Moeller A,
Bordignon E ,
Seeger MA
(2022) Sci Adv , 8 , eabn6845 - eabn6845
Grant Support:
- German Research Foundation (DFG): CRC944 (Germany)
- German Research Foundation (DFG): SCHA 1574/6-1 (Germany)
- German Research Foundation (DFG): EXC 2033 - 390677874 - RESOLV (Germany)