EMDB EMD-12893

Single particle reconstruction
3.0Å resolution

Drs2p-Cdc50p in the E1 state

Map released:
Overview of EMD-12893
Fitted atomic model: 7oh4
Primary publication:
Structural basis of substrate-independent phosphorylation in a P4-ATPase lipid flippase
Timcenko M, Dieudonne T, Montigny C, Boesen T, Lyons JA, Lenoir G, Nissen P
J.Mol.Biol. 167062- (2021)
PMID: 34023399

Function and Biology Details

Sample name: Binary complex of Drs2p-Cdc50p with the regulatory lipid PI4P
Ligands: (2R)-1-{[(R)-hydroxy{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}-3-(octadecanoyloxy)propan-2-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate, MAGNESIUM ION, 2-acetamido-2-deoxy-beta-D-glucopyranose, water
Proteins: Binary complex of Drs2p-Cdc50p with the regulatory lipid PI4P, Drs2p, Cdc50p, Probable phospholipid-transporting ATPase DRS2,Probable phospholipid-transporting ATPase DRS2, Cell division control protein 50

Experimental Information Details

Resolution: 3.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: OTHER