EMDB EMD-12586

Single particle reconstruction
3.5Å resolution

Trimeric SARS-CoV-2 spike ectodomain in complex with biliverdin (one RBD erect)

Map released:
Last modified:
Overview of EMD-12586
Fitted atomic model: 7nta
Primary publication:
SARS-CoV-2 can recruit a haem metabolite to evade antibody immunity.
Rosa A, Pye VE, Graham C, Muir L, Seow J, Ng KW, Cook NJ, Rees-Spear C, Parker E, Silva Dos Santos M, Rosadas C, Susana A, Rhys H, Nans A, Masino L, Roustan C, Christodoulou E, Ulferts R, Wrobel AG, Short CE, Fertleman M, Sanders RW, Heaney J, Spyer M, Kjaer S, Riddell A, Malim MH, Beale R, MacRae JI, Taylor GP, Nastouli E, van Gils MJ, Rosenthal PB, Pizzato M, McClure MO, Tedder RS, Kassiotis G, McCoy LE, Doores KJ, Cherepanov P
Sci Adv - (2021)
PMID: 33888467

Function and Biology Details

Sample name: Ectodomain of S1 protein from SARS-CoV-2 expressed in HEK cells and purified. Sample in the presence of excess biliverdin.
Ligands: BILIVERDINE IX ALPHA, 2-acetamido-2-deoxy-beta-D-glucopyranose, water
Proteins: Ectodomain of S1 protein from SARS-CoV-2 expressed in HEK cells and purified. Sample in the presence of excess biliverdin., Spike glycoprotein

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)