EMDB EMD-12585

Single particle reconstruction
3.36Å resolution

Trimeric SARS-CoV-2 spike ectodomain in complex with biliverdin (closed conformation)

Map released:
Last modified:
Overview of EMD-12585
Fitted atomic model: 7nt9
Primary publication:
SARS-CoV-2 can recruit a haem metabolite to evade antibody immunity.
Rosa A, Pye VE, Graham C, Muir L, Seow J, Ng KW, Cook NJ, Rees-Spear C, Parker E, Silva Dos Santos M, Rosadas C, Susana A, Rhys H, Nans A, Masino L, Roustan C, Christodoulou E, Ulferts R, Wrobel AG, Short CE, Fertleman M, Sanders RW, Heaney J, Spyer M, Kjaer S, Riddell A, Malim MH, Beale R, MacRae JI, Taylor GP, Nastouli E, van Gils MJ, Rosenthal PB, Pizzato M, McClure MO, Tedder RS, Kassiotis G, McCoy LE, Doores KJ, Cherepanov P
Sci Adv - (2021)
PMID: 33888467

Function and Biology Details

Sample name: Ectodomain of spike glycoprotein from SARS-CoV-2 expressed in HEK cells and purified. Data were collected in the presence of excess biliverdin.
Ligands: BILIVERDINE IX ALPHA, 2-acetamido-2-deoxy-beta-D-glucopyranose, water
Proteins: Ectodomain of spike glycoprotein from SARS-CoV-2 expressed in HEK cells and purified. Data were collected in the presence of excess biliverdin., Spike glycoprotein

Experimental Information Details

Resolution: 3.36Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C3
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)