EMDB EMD-12040

Single particle reconstruction
3.91Å resolution

Ubiquitin ligation to F-box protein substrates by SCF-RBR E3-E3 super-assembly: CUL1-RBX1-SKP1-SKP2-CKSHS1-p27~Ub~ARIH1. Transition State 2

Map released:
Last modified:
Overview of EMD-12040
Source organism: Homo sapiens [9606]
Fitted atomic model: 7b5m
Primary publication:
Ubiquitin ligation to F-box protein targets by SCF-RBR E3-E3 super-assembly.
Horn-Ghetko D, Krist DT, Prabu JR, Baek K, Mulder MPC, Klugel M, Scott DC, Ovaa H, Kleiger G, Schulman BA
Nature - (2021)
PMID: 33536622

Function and Biology Details

Sample name: CUL1-RBX1-SKP1-SKP2-CKSHS1-p27~Ub~ARIH1
Ligand: ZINC ION
Proteins: CUL1-RBX1-SKP1-SKP2-CKSHS1-p27~Ub~ARIH1, Cullin-1, E3 ubiquitin-protein ligase RBX1, ARIH1, SKP2, CKS1B, SKP1, UBC, Cyclin-dependent kinase inhibitor 1B, Cullin-1, E3 ubiquitin-protein ligase ARIH1, S-phase kinase-associated protein 2, Cyclin-dependent kinases regulatory subunit 1, S-phase kinase-associated protein 1, Polyubiquitin-C, E3 ubiquitin-protein ligase RBX1, Cyclin-dependent kinase inhibitor 1B

Experimental Information Details

Resolution: 3.91Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: OTHER