EMDB EMD-11981

Single particle reconstruction
2.62Å resolution

SARS-CoV-spike bound to two neutralising nanobodies

Map released:
Overview of EMD-11981
Fitted atomic model: 7b18
Primary publication:
Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape.
Koenig PA, Das H, Liu H, Kummerer BM, Gohr FN, Jenster LM, Schiffelers LDJ, Tesfamariam YM, Uchima M, Wuerth JD, Gatterdam K, Ruetalo N, Christensen MH, Fandrey CI, Normann S, Todtmann JMP, Pritzl S, Hanke L, Boos J, Yuan M, Zhu X, Schmid-Burgk JL, Kato H, Schindler M, Wilson IA, Geyer M, Ludwig KU, Hallberg BM, Wu NC, Schmidt FI
Science 371 - (2021)
PMID: 33436526

Function and Biology Details

Sample name: SARS-CoV-2 glycoprotein in complex with two nanobodys named V
Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
Proteins: SARS-CoV-2 glycoprotein in complex with two nanobodys named V, Spike glycoprotein, Nanobody against SARS-CoV-2 VHH E, Nanobody against spike glycoprotein VHH V, Spike glycoprotein, Nanobody against SARS-CoV-2 VHH E, Nanobody against spike glycoprotein VHH V

Experimental Information Details

Resolution: 2.62Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: OTHER