EMDB EMD-11972

Single particle reconstruction
3.6Å resolution

Overall map of a transcribing RNA polymerase II-U1 snRNP complex with 145-nt RNA

Map released:
Last modified:
Overview of EMD-11972
Source organisms:
Fitted atomic model: 7b0y
Primary publication:
Structure of a transcribing RNA polymerase II-U1 snRNP complex.
Zhang S, Aibara S, Vos SM, Agafonov DE, Luhrmann R, Cramer P
Science 371 305-309 (2021)
PMID: 33446560

Function and Biology Details

Sample name: The transcribing RNA polymerase II-U1 snRNP complex with 145-nt RNA
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: Non-template DNA, 145-nt RNA, Template DNA, U1 snRNA
Proteins: The transcribing RNA polymerase II-U1 snRNP complex with 145-nt RNA, RNA polymerase II, Nucleic acids, U1 snRNP, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase II subunit RPB3, DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerase II subunit F, DNA-directed RNA polymerase II subunit RPB7, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11-a, RPB12, U1 small nuclear ribonucleoprotein 70 kDa, U1 small nuclear ribonucleoprotein A, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein-associated protein, Small nuclear ribonucleoprotein Sm D1

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: OTHER