EMDB EMD-11846

Single particle reconstruction
3.5Å resolution

mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.

Map released:
Overview of EMD-11846
Fitted atomic model: 7aor
Primary publication:
Structure of the mature kinetoplastids mitoribosome and insights into its large subunit biogenesis.
Soufari H, Waltz F, Parrot C, Durrieu-Gaillard S, Bochler A, Kuhn L, Sissler M, Hashem Y
Proc.Natl.Acad.Sci.USA 117 29851-29861 (2020)
PMID: 33168716

Function and Biology Details

Sample name: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3.
Ligands: GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, URIDINE 5'-TRIPHOSPHATE, ZINC ION
Nucleic acid: RNA (478-MER)
Proteins: mt-SSU from Trypanosoma cruzi in complex with mt-IF-3., mt-SSU from Trypanosoma cruzi in complex with mt-IF-3., mt-SSU from Trypanosoma cruzi in complex with mt-IF-3., mt-SSU from Trypanosoma cruzi in complex with mt-IF-3., mS35, mS33, mS29, uS19, uS14m, uS10m, mS72, mS71, mS70, mS69, Rhodanese domain-containing protein, mS58, mS57, mS55, mS54, mS53, mS52, mS50, mS49, mS51, bS18m, uS15m, uS11m, uS8m, uS9m, mS73, mS68, mS66, AKAP7_NLS domain-containing protein, mS63, mS62, mS61, mS56, mS60, mS47, Sod_Fe_C domain-containing protein, Superoxide dismutase, putative, Protein FYV4, mitochondrial, mS37, mS38, Trafficking protein particle complex subunit, mS59, mS26, mS23, mS22, bS21m, bS16m, bS6m, MURF5, Ribosomal_S5_C domain-containing protein, mt-iF3, mS48, 30S Ribosomal protein S17-like protein, Ribosome protein S12, mS65

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TALOS ARCTICA
Detector: FEI FALCON III (4k x 4k)