EMDB EMD-11822

Single particle reconstruction
3.5Å resolution

RET/GDNF/GFRa1 extracellular complex Cryo-EM structure

Map released:
Overview of EMD-11822
Source organism: Danio rerio [7955]
Fitted atomic model: 7aml
Related EM entry by publication: EMD-11777
Primary publication:
A two-site flexible clamp mechanism for RET-GDNF-GFRa1 assembly reveals both conformational adaptation and strict geometric spacing
Adams SE, Purkiss AG, Knowles PK, Nans A, Briggs DC, Borg A, Earl CP, Goodman KM, Narowtek A, McIntosh PJ, Houghton FM, Kjaer S, McDonald NQ
Structure 29 - (2021)

Function and Biology Details

Sample name: Ternary complex of RET extracellular domain with GDNF and GFRa1 ligand-coreceptor pair
Ligands: CALCIUM ION, 2-acetamido-2-deoxy-beta-D-glucopyranose
Proteins: Ternary complex of RET extracellular domain with GDNF and GFRa1 ligand-coreceptor pair, Proto-oncogene tyrosine-protein kinase receptor Ret, GDNF family receptor alpha, Glial cell line-derived neurotrophic factor

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)