EMDB EMD-11777

Single particle reconstruction
26.0Å resolution

RET/GDF15/GFRAL extracellular complex negative stain envelope

Map released:
Overview of EMD-11777
Source organism: Homo sapiens [9606]
Related EM entry by publication: EMD-11822
Primary publication:
A two-site flexible clamp mechanism for RET-GDNF-GFRa1 assembly reveals both conformational adaptation and strict geometric spacing
Adams SE, Purkiss AG, Knowles PK, Nans A, Briggs DC, Borg A, Earl CP, Goodman KM, Narowtek A, McIntosh PJ, Houghton FM, Kjaer S, McDonald NQ
Structure 29 - (2021)

Function and Biology Details

Sample name: Ternary complex of the human RET extracellular domain with the GDF15/GFRAL ligand-co-receptor pair
Proteins: Ternary complex of the human RET extracellular domain with the GDF15/GFRAL ligand-co-receptor pair, Proto-oncogene tyrosine-protein kinase receptor Ret, GDNF family receptor alpha-like, Growth/differentiation factor 15

Experimental Information Details

Resolution: 26.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TECNAI SPIRIT
Detector: GATAN ULTRASCAN 1000 (2k x 2k)