EMDB EMD-1172

Single particle reconstruction
11.5Å resolution

Conformational transition of initiation factor 2 from the GTP- to GDP-bound state visualized on the ribosome.

Map released:
Last modified:
Overview of EMD-1172
Source organism: Thermus thermophilus HB8 [300852]
Related EM entry by publication: EMD-1173
Primary publication:
Conformational transition of initiation factor 2 from the GTP- to GDP-bound state visualized on the ribosome.
Myasnikov AG, Marzi S, Simonetti A, Giuliodori AM, Gualerzi CO, Yusupova G, Yusupov M, Klaholz BP
NAT.STRUCT.MOL.BIOL. 12 1145-1149 (2005)
PMID: 16284619

Function and Biology Details

Sample name: Ribosome complex with initiation factor 2 in GTP state
Nucleic acids: messenger RNA, formyl-methionyl-RNA
Protein: initiation factor 2
Ribosome: 70S

Experimental Information Details

Resolution: 11.5Å
Resolution method: FSC 0.143
Applied symmetry: C1
Reconstruction software: IMAGIC
Microscope: FEI TECNAI F20
Detector: KODAK SO-163 FILM