EMDB EMD-11666

Single particle reconstruction
3.9Å resolution

Nanodisc reconstituted, drug-free human ABCB1 in complex with MRK16 Fab

Map released:
Last modified:
Overview of EMD-11666
Source organisms:
Fitted atomic model: 7a65
Related EM entries by publication: EMD-11667, EMD-11670, EMD-11671, EMD-11672
Primary publication:
Cryo-EM structures reveal distinct mechanisms of inhibition of the human multidrug transporter ABCB1.
Nosol K, Romane K, Irobalieva RN, Alam A, Kowal J, Fujita N, Locher KP
Proc.Natl.Acad.Sci.USA 117 26245-26253 (2020)
PMID: 33020312

Function and Biology Details

Sample name: Nanodisc reconstituted, drug-free human ABCB1 in complex with MRK16 Fab
Ligand: CHOLESTEROL
Proteins: Nanodisc reconstituted, drug-free human ABCB1 in complex with MRK16 Fab, Multidrug resistance protein 1, MRK16 Fab-fragment light and heavy chain, Multidrug resistance protein 1, MRK16 Fab-fragment light chain, MRK16 Fab-fragment heavy chain

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)