EMDB EMD-11657

Single particle reconstruction
1.7Å resolution

CryoEM structure of a human gamma-aminobutyric acid receptor, the GABA(A)R-beta3 homopentamer, in complex with histamine and megabody Mb25 in lipid nanodisc

Map released:
Last modified:
Overview of EMD-11657
Source organisms:
Fitted atomic model: 7a5v
Related EM entry by publication: EMD-11638
Primary publication:
Single-particle cryo-EM at atomic resolution.
Nakane T, Kotecha A, Sente A, McMullan G, Masiulis S, Brown PMGE, Grigoras IT, Malinauskaite L, Malinauskas T, Miehling J, Uchanski T, Yu L, Karia D, Pechnikova EV, de Jong E, Keizer J, Bischoff M, McCormack J, Tiemeijer P, Hardwick SW, Chirgadze DY, Murshudov G, Aricescu AR, Scheres SHW
Nature 587 152-156 (2020)
PMID: 33087931

Function and Biology Details

Sample name: Human GABA(A)R-beta3 homopentamer in complex with Megabody-25 in lipid nanodisc
Ligands: HEXADECANE, DECANE, 2-acetamido-2-deoxy-beta-D-glucopyranose, N-OCTANE, HISTAMINE, CHLORIDE ION, water
Proteins: Human GABA(A)R-beta3 homopentamer in complex with Megabody-25 in lipid nanodisc, Human GABA(A)R-beta3 homopentamer, Megabody-25, Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3, Megabody Mb25

Experimental Information Details

Resolution: 1.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C5
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: OTHER