EMDB EMD-11569

Single particle reconstruction
3.4Å resolution

Human C Complex Spliceosome - High-resolution CORE

Map released:
Overview of EMD-11569
Source organism: Human [9606]
Fitted atomic model: 6zym
Related EM entries by publication: EMD-11570, EMD-11571, EMD-11572, EMD-11573, EMD-11574
Primary publication:
Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome.
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol.Cell 80 127-139.e6 (2020)
PMID: 33007253

Function and Biology Details

Sample name: Human C complex Spliceosome - High resolution core
Ligands: MAGNESIUM ION, INOSITOL HEXAKISPHOSPHATE, GUANOSINE-5'-TRIPHOSPHATE, ZINC ION
Nucleic acids: U2 snRNA, U5 snRNA, U6 snRNA, pre-mRNA
Proteins: Human C complex Spliceosome - High resolution core, Corepressor interacting with RBPJ 1, Pre-mRNA-processing-splicing factor 8, 116 kDa U5 small nuclear ribonucleoprotein component, SNW domain-containing protein 1, Pleiotropic regulator 1, Pre-mRNA-processing factor 17, U5 small nuclear ribonucleoprotein 40 kDa protein, Cell division cycle 5-like protein, Crooked neck-like protein 1, Pre-mRNA-splicing factor RBM22, Protein BUD31 homolog, Spliceosome-associated protein CWC15 homolog, Serine/arginine repetitive matrix protein 2, Pre-mRNA-splicing factor CWC22 homolog, Peptidyl-prolyl cis-trans isomerase-like 1, WD repeat-containing protein 70, Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16, Pre-mRNA-splicing factor ISY1 homolog, Pre-mRNA-splicing factor CWC25 homolog, Splicing factor YJU2, Protein FRG1

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)