EMD-11488

Single-particle
3.2 Å
EMD-11488
3D View Gallery
Deposition: 28/07/2020
Map released: 18/11/2020
Last modified: 09/04/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-11488
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6zwm summary report (pdf.gz) Map and 6zwm FULL report (pdf.gz)
EMICSS annotations (xml)

cryo-EM structure of human mTOR complex 2, overall refinement

EMD-11488

Single-particle
3.2 Å
EMD-11488
3D View Gallery
Deposition: 28/07/2020
Map released: 18/11/2020
Last modified: 09/04/2025
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.37 mg/mL
Buffer
pH: 8.5
Grid
Mesh: 400
Model: Quantifoil
Material: COPPER
Support Film [2]
Material Topology Thickness
CARBON HOLEY -
CARBON CONTINUOUS -
Vitrification
Cryogen name: ETHANE-PROPANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 100.0 µm
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 70.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 293038
Startup model [1]
Type: OTHER
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 320 320 320
Dimensions (Å) 430.08 430.08 430.08
Voxel size (Å) 1.344 1.344 1.344
Contour list
Primary Level Source
True 0.375 AUTHOR