EMDB EMD-11420

Single particle reconstruction
3.3Å resolution

E. coli 70S-RNAP expressome complex in collided state without NusG

Map released:
Last modified:
Overview of EMD-11420
Source organisms:
Fitted atomic model: 6ztm
Related EM entries by publication: EMD-11418, EMD-11419, EMD-11421, EMD-11422, EMD-11423, EMD-11426
Primary publication:
Structural basis of transcription-translation coupling and collision in bacteria.
Webster MW, Takacs M, Zhu C, Vidmar V, Eduljee A, Abdelkareem M, Weixlbaumer A
Science 369 1355-1359 (2020)
PMID: 32820062

Function and Biology Details

Sample name: Uncoupled E. coli expressome, state 1
Ligands: MAGNESIUM ION, PHENYLALANINE, ZINC ION
Nucleic acids: 16S ribosomal RNA, mRNA, tRNA(fmet) P-site, Phe-NH-tRNA(Phe) A-site, 23S ribosomal RNA, 5S ribosomal RNA, Non-template DNA strand, Template DNA strand
Proteins: Uncoupled E. coli expressome, state 1, 70S ribosome within the E. coli 70S-RNAP expressome complex in collided state without NusG, DNA-directed RNA polymerase within the E. coli 70S-RNAP expressome complex in collided state without NusG, synthetic mRNA and template DNA strands, 30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18, 30S ribosomal protein S19, 30S ribosomal protein S20, 30S ribosomal protein S21, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta'

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)