EMDB EMD-11390

Single particle reconstruction
4.0Å resolution

Human mitochondrial ribosome in complex with ribosome recycling factor

Map released:
Overview of EMD-11390
Source organism: Human [9606]
Fitted atomic model: 6zs9
Related EM entries by publication: EMD-11391, EMD-11392, EMD-11393, EMD-11394, EMD-11395, EMD-11397
Primary publication:
Structural basis of mitochondrial translation.
Aibara S, Singh V, Modelska A, Amunts A
Elife 9 - (2020)
PMID: 32812867

Function and Biology Details

Sample name: Human mitochondrial ribosome bound to mRNA and A-, P- and E-site tRNAs
Ligands: ZINC ION, MAGNESIUM ION, ~{N}-[(3~{S},6~{R},12~{R},15~{S},16~{S},19~{S},22~{S},25~{S})-25-[[(3~{S})-1-azabicyclo[2.2.2]octan-3-yl]sulfanylmethyl]-3-[[4-(dimethylamino)phenyl]methyl]-12-ethyl-4,16-dimethyl-2,5,11,14,18,21,24-heptakis(oxidanylidene)-19-phenyl-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide, 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE, GUANOSINE-5'-TRIPHOSPHATE
Nucleic acids: 16S rRNA, 12S rRNA, mt-tRNAVal
Proteins: Human mitochondrial ribosome bound to mRNA and A-, P- and E-site tRNAs, 39S ribosomal protein L32, mitochondrial, 39S ribosomal protein L33, mitochondrial, 39S ribosomal protein L34, mitochondrial, 39S ribosomal protein L35, mitochondrial, 39S ribosomal protein L36, mitochondrial, 39S ribosomal protein L37, mitochondrial, 39S ribosomal protein L38, mitochondrial, 39S ribosomal protein L39, mitochondrial, 39S ribosomal protein L40, mitochondrial, 39S ribosomal protein L41, mitochondrial, 28S ribosomal protein S34, mitochondrial, 28S ribosomal protein S35, mitochondrial, Coiled-coil-helix-coiled-coil-helix domain-containing protein 1, Aurora kinase A-interacting protein, Pentatricopeptide repeat domain-containing protein 3, mitochondrial, Ribosome-recycling factor, mitochondrial, 28S ribosomal protein S2, mitochondrial, 28S ribosomal protein S24, mitochondrial, 28S ribosomal protein S5, mitochondrial, 28S ribosomal protein S6, mitochondrial, 28S ribosomal protein S7, mitochondrial, 28S ribosomal protein S9, mitochondrial, 28S ribosomal protein S10, mitochondrial, 28S ribosomal protein S11, mitochondrial, 28S ribosomal protein S12, mitochondrial, 28S ribosomal protein S14, mitochondrial, 28S ribosomal protein S15, mitochondrial, 28S ribosomal protein S16, mitochondrial, 28S ribosomal protein S17, mitochondrial, 28S ribosomal protein S18b, mitochondrial, 28S ribosomal protein S18c, mitochondrial, 28S ribosomal protein S21, mitochondrial, 28S ribosomal protein S22, mitochondrial, 28S ribosomal protein S23, mitochondrial, 28S ribosomal protein S25, mitochondrial, 28S ribosomal protein S26, mitochondrial, 28S ribosomal protein S27, mitochondrial, 28S ribosomal protein S28, mitochondrial, 28S ribosomal protein S29, mitochondrial, 28S ribosomal protein S31, mitochondrial, 28S ribosomal protein S33, mitochondrial, 39S ribosomal protein L2, mitochondrial, 39S ribosomal protein L3, mitochondrial, 39S ribosomal protein L4, mitochondrial, 39S ribosomal protein L9, mitochondrial, 39S ribosomal protein L10, mitochondrial, 39S ribosomal protein L11, mitochondrial, 39S ribosomal protein L13, mitochondrial, 39S ribosomal protein L14, mitochondrial, 39S ribosomal protein L15, mitochondrial, 39S ribosomal protein L16, mitochondrial, 39S ribosomal protein L17, mitochondrial, Mitochondrial ribosomal protein L18, isoform CRA_b, 39S ribosomal protein L19, mitochondrial, 39S ribosomal protein L20, mitochondrial, 39S ribosomal protein L21, mitochondrial, 39S ribosomal protein L22, mitochondrial, 39S ribosomal protein L23, mitochondrial, 39S ribosomal protein L24, mitochondrial, 39S ribosomal protein L27, mitochondrial, 39S ribosomal protein L28, mitochondrial, 39S ribosomal protein L47, mitochondrial, 39S ribosomal protein L30, mitochondrial, 39S ribosomal protein L42, mitochondrial, 39S ribosomal protein L43, mitochondrial, 39S ribosomal protein L44, mitochondrial, 39S ribosomal protein L45, mitochondrial, 39S ribosomal protein L46, mitochondrial, 39S ribosomal protein L48, mitochondrial, 39S ribosomal protein L49, mitochondrial, 39S ribosomal protein L50, mitochondrial, 39S ribosomal protein L51, mitochondrial, cDNA FLJ76418, highly similar to Homo sapiens mitochondrial ribosomal protein L52 (MRPL52), transcript variant 1, mRNA, 39S ribosomal protein L53, mitochondrial, 39S ribosomal protein L54, mitochondrial, 39S ribosomal protein L55, mitochondrial, Ribosomal protein 63, mitochondrial, Peptidyl-tRNA hydrolase ICT1, mitochondrial, Growth arrest and DNA damage-inducible proteins-interacting protein 1, 39S ribosomal protein S18a, mitochondrial, 39S ribosomal protein S30, mitochondrial, 39S ribosomal protein L12, mitochondrial

Experimental Information Details

Resolution: 4.0Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN
Detector: GATAN K2 SUMMIT (4k x 4k)