EMDB EMD-11335

Single particle reconstruction
2.9Å resolution

SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 2

Map released:
Last modified:
Overview of EMD-11335
Source organisms:
Fitted atomic model: 6zp4
Primary publication:
Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2.
Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, Kratzat H, Hayn M, Mackens-Kiani T, Cheng J, Straub JH, Sturzel CM, Frohlich T, Berninghausen O, Becker T, Kirchhoff F, Sparrer KMJ, Beckmann R
Science 369 1249-1255 (2020)
PMID: 32680882

Function and Biology Details

Sample name: SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 2
Ligands: ZINC ION, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Nucleic acids: 18S ribosomal RNA, tRNA
Proteins: SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 2, human 43S preinitiation ribosome complex - state 2, SARS-CoV-2 Nsp1, 40S ribosomal protein SA, 40S ribosomal protein S3a, 40S ribosomal protein S2, 40S ribosomal protein S26, 40S ribosomal protein S4, X isoform, 60S ribosomal protein L41, 40S ribosomal protein S6, 40S ribosomal protein S7, 40S ribosomal protein S8, 40S ribosomal protein S9, 40S ribosomal protein S11, 40S ribosomal protein S13, 40S ribosomal protein S21, 40S ribosomal protein S15a, 40S ribosomal protein S24, 40S ribosomal protein S27, 40S ribosomal protein S30, 40S ribosomal protein S17, 40S ribosomal protein S3, 40S ribosomal protein S5, 40S ribosomal protein S10, 40S ribosomal protein S12, 40S ribosomal protein S15, 40S ribosomal protein S16, 40S ribosomal protein S18, 40S ribosomal protein S19, 40S ribosomal protein S20, 40S ribosomal protein S25, 40S ribosomal protein S28, 40S ribosomal protein S29, Ubiquitin-40S ribosomal protein S27a, Receptor of activated protein C kinase 1, 40S ribosomal protein S14, 40S ribosomal protein S23, Eukaryotic translation initiation factor 1A, X-chromosomal, Eukaryotic translation initiation factor 3 subunit I, Eukaryotic translation initiation factor 3 subunit B, Eukaryotic translation initiation factor 3 subunit A, Eukaryotic translation initiation factor 3 subunit C, Eukaryotic translation initiation factor 3 subunit E, Eukaryotic translation initiation factor 3 subunit F, Eukaryotic translation initiation factor 3 subunit H, Eukaryotic translation initiation factor 3 subunit K, Eukaryotic translation initiation factor 3 subunit L, Eukaryotic translation initiation factor 3 subunit M, Eukaryotic translation initiation factor 3 subunit D, Unknown factor, Eukaryotic translation initiation factor 2 subunit 2, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit 3, Eukaryotic translation initiation factor 1, Non-structural protein 1

Experimental Information Details

Resolution: 2.9Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)