EMDB EMD-11247

Single particle reconstruction
2.8Å resolution

Complex I during turnover, open3

Map released:
Overview of EMD-11247
Source organism: Sheep [9940]
Fitted atomic model: 6zkf
Primary publication:
The coupling mechanism of mammalian respiratory complex I.
Kampjut D, Sazanov LA
Science - (2020)
PMID: 32972993

Function and Biology Details

Sample name: Complex I during turnover, open3
Ligands: IRON/SULFUR CLUSTER, FLAVIN MONONUCLEOTIDE, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, FE2/S2 (INORGANIC) CLUSTER, POTASSIUM ION, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE, 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione, CARDIOLIPIN, S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate, ZINC ION, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ADENOSINE MONOPHOSPHATE, MYRISTIC ACID
Proteins: Complex I during turnover, open3, NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial, Mitochondrial complex I, 24 kDa subunit, NADH:ubiquinone oxidoreductase core subunit S1, Mitochondrial complex I, 49 kDa subunit, NADH:ubiquinone oxidoreductase core subunit S3, Mitochondrial complex I, PSST subunit, Mitochondrial complex I, TYKY subunit, NADH-ubiquinone oxidoreductase chain 3, NADH-ubiquinone oxidoreductase chain 1, NADH-ubiquinone oxidoreductase chain 6, NADH-ubiquinone oxidoreductase chain 4L, NADH-ubiquinone oxidoreductase chain 5, NADH-ubiquinone oxidoreductase chain 4, NADH-ubiquinone oxidoreductase chain 2, Mitochondrial complex I, B14.7 subunit, NADH:ubiquinone oxidoreductase subunit B5, Acyl carrier protein, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, Mitochondrial complex I, PDSW subunit, Mitochondrial complex I, 10 kDa subunit, Mitochondrial complex I, 13 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, NADH:ubiquinone oxidoreductase subunit A9, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2, Mitochondrial complex I, B13 subunit, NADH:ubiquinone oxidoreductase subunit A6, Mitochondrial complex I, B14.5a subunit, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, NADH:ubiquinone oxidoreductase subunit S5, NADH:ubiquinone oxidoreductase subunit A3, NADH:ubiquinone oxidoreductase subunit B3, NADH dehydrogenase [ubiquinone] 1 subunit C2, NADH:ubiquinone oxidoreductase subunit B4, Mitochondrial complex I, B16.6 subunit, Mitochondrial complex I, B17 subunit, NADH:ubiquinone oxidoreductase subunit B7, NADH:ubiquinone oxidoreductase subunit B9, NADH:ubiquinone oxidoreductase subunit B2, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial, Mitochondrial complex I, ESSS subunit, Mitochondrial complex I, KFYI subunit, Mitochondrial complex I, MNLL subunit, Mitochondrial complex I, MWFE subunit

Experimental Information Details

Resolution: 2.8Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)