EMDB EMD-11118

Single particle reconstruction
3.02Å resolution

La Crosse virus polymerase at elongation mimicking stage

Map released:
Overview of EMD-11118
Source organism: La Crosse orthobunyavirus [2560547]
Fitted atomic model: 6z8k
Related EM entries by publication: EMD-11093, EMD-11095, EMD-11107
Primary publication:
Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.
Arragain B, Effantin G, Gerlach P, Reguera J, Schoehn G, Cusack S, Malet H
Nat Commun 11 3590-3590 (2020)
PMID: 32681014

Function and Biology Details

Sample name: La Crosse virus polymerase at elongation-mimicking stage
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: La Crosse virus 5' vRNA 1-10, La Crosse virus 5' vRNA (9-16), La Crosse virus 3' vRNA (1-16)
Proteins: La Crosse virus polymerase at elongation-mimicking stage, La Crosse virus polymerase at elongation stage, RNA-directed RNA polymerase L, RNA-directed RNA polymerase L

Experimental Information Details

Resolution: 3.02Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)