EMDB EMD-11063

Single particle reconstruction
2.97Å resolution

Structure of SMG1-8-9 kinase complex bound to UPF1-LSQ

Map released:
Overview of EMD-11063
Source organism: Homo sapiens [9606]
Fitted atomic model: 6z3r
Primary publication:
Structure of substrate-bound SMG1-8-9 kinase complex reveals molecular basis for phosphorylation specificity.
Langer LM, Gat Y, Bonneau F, Conti E
Elife 9 - (2020)
PMID: 32469312

Function and Biology Details

Sample name: SMG1-8-9 bound to AMPPNP and UPF1-LSQ
Ligands: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, INOSITOL HEXAKISPHOSPHATE, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Proteins: SMG1-8-9 bound to AMPPNP and UPF1-LSQ, SMG1-8-9, UPF1-LSQ, Serine/threonine-protein kinase SMG1,Serine/threonine-protein kinase SMG1,SMG1,Serine/threonine-protein kinase SMG1,Serine/threonine-protein kinase SMG1,Serine/threonine-protein kinase SMG1, Protein SMG8, Protein SMG9, Regulator of nonsense transcripts 1

Experimental Information Details

Resolution: 2.97Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: OTHER