EMDB EMD-11032

Single particle reconstruction
3.7Å resolution

The mitochondrial ribosome from Tetrahymena thermophila

Map released:
Overview of EMD-11032
Source organism: Tetrahymena thermophila (strain SB210) [312017]
Fitted atomic model: 6z1p
To be published

Function and Biology Details

Sample name: The mitochondrial ribosome from tetrahymena thermophila
Ligands: MAGNESIUM ION, ZINC ION, FE2/S2 (INORGANIC) CLUSTER, ADENOSINE-5'-TRIPHOSPHATE
Nucleic acids: LSU rRNA_1, LSU rRNA_2, SSU rRNA_1, SSU rRNA_2
Proteins: The mitochondrial ribosome from tetrahymena thermophila, Ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, Ymf69, Ymf60, bL7/L12m, bL7/12m, bL7/12m, Ribosomal protein L7/L12 carboxy-terminal domain protein, Ribosomal protein L9, amine-terminal domain protein, Ymf74, Ribosomal protein L11, amine-terminal domain protein, 50S ribosomal protein L13, Ribosomal protein L14, Ribosomal protein L15, putative, Ribosomal protein L16, 50S ribosomal protein L17, bL19m, 50S ribosomal protein L20, 50S ribosomal protein L21, Ribosomal protein L22/L17e, Ribosomal protein L23, putative, 50S ribosomal protein L24, bL25m, 50S ribosomal protein L27, Ribosomal protein L28, 39-S ribosomal protein L47, bL32m, 50S ribosomal protein L33, bL35m, Ribosomal protein, mL40, Ribosomal protein L27, Ribosomal protein L51/S25/CI-B8 domain protein, mL46, Large subunit ribosomal protein, mL53, mL54, mL64, mL101, mL102, mL103, mL104, Signal peptide-binding domain protein, mL106, Iron-binding zinc finger CDGSH type protein, Ymf73, Ymf64, Ymf76, 30S ribosomal protein S5, Ribosomal protein S6, Ymf63, Ribosomal protein S9, Ymf59, Ymf61, Ribosomal protein S12, Ribosomal protein S13, Ribosomal protein S14, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, Ribosomal protein S18, Ribosomal protein S19, bS21m, mS23, mS26, Ribosomal death-associated protein, mS31, mS33, mS34, Ribosomal subunit protein, mS37, mS38, IGR motif protein, mS45, mS75, mS76, mS77, Enoyl-CoA hydratase/isomerase, mS78, mS79, SelR domain protein, mS81, mS82, PARP alpha-helical domain-containing protein,mS83, mS84,mS84, mS85, Iron donor protein CyaY, mS87, mS88, mS89, mS90, mS91, Ribosomal protein S3, mS93

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)