EMDB EMD-11000

Single particle reconstruction
3.1Å resolution

State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate

Map released:
Overview of EMD-11000
Source organism: Trypanosoma brucei brucei [5702]
Fitted atomic model: 6yxy
Related EM entry by publication: EMD-10999
Primary publication:
Structural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis.
Jaskolowski M, Ramrath DJF, Bieri P, Niemann M, Mattei S, Calderaro S, Leibundgut M, Horn EK, Boehringer D, Schneider A, Ban N
Mol.Cell 79 629-644.e4 (2020)
PMID: 32679035

Function and Biology Details

Sample name: State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
Ligands: ZINC ION, MAGNESIUM ION, SPERMIDINE, GUANOSINE-5'-TRIPHOSPHATE, SODIUM ION, ADENOSINE-5'-TRIPHOSPHATE, S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, water
Nucleic acid: 12S ribosomal RNA
Proteins: State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate, bL28m, uL29m, uL30m, bL32m, bL35m, mL67, mt-EngA, mL68, mt-LAF2, mt-LAF3, mL70, mt-LAF4, uL3m, mL71, mt-LAF5, UNK, uL4m, mL72, mt-LAF6, mt-LAF7, mL74, mt-LAF8, bL9m, mL75, MALSU1, UNK, mL76, L0R8F8, uL11m, mL77, mt-ACP, UNK, mL78, mt-LAF12, UNK, Mtg1, UNK, uL13m, mL80, mt-LAF14, uL14m, mL81, mt-LAF15a, uL15m, mL83, mt-EngB, bL17m, mL84, mL85, mt-LAF18, bL19m, mL86, mt-LAF19, bL20m, mL87, mt-LAF20, bL21m, mL88, uL22m, mL89, uL23m, mL90, UNK, uL24m, mL92, mL93, mL94, mL95, mL41, mL42, mL98, mL43, mL99, mL100, mL101, mL49, mL52, mL53, mL63, mL64

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)