EMDB EMD-10999

Single particle reconstruction
3.9Å resolution

State A of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate

Map released:
Overview of EMD-10999
Source organism: Trypanosoma brucei brucei [5702]
Fitted atomic model: 6yxx
Related EM entry by publication: EMD-11000
Primary publication:
Structural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis.
Jaskolowski M, Ramrath DJF, Bieri P, Niemann M, Mattei S, Calderaro S, Leibundgut M, Horn EK, Boehringer D, Schneider A, Ban N
Mol.Cell 79 629-644.e4 (2020)
PMID: 32679035

Function and Biology Details

Sample name: State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
Ligands: ZINC ION, MAGNESIUM ION, GUANOSINE-5'-TRIPHOSPHATE, SODIUM ION, ADENOSINE-5'-TRIPHOSPHATE, S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, water
Nucleic acid: 12S ribosomal RNA
Proteins: State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate, bL28m, mt-LAF21, uL29m, DUF4379 domain-containing protein, uL30m, mt-LAF23, mt-LAF24, bL32m, mt-LAF25, KRIPP3, bL35m, mL67, mt-EngA, UNK, mL68, DEAD-box helicase, putative, Pseudouridylate synthase, putative, mL70, mt-LAF4, Ribosomal protein L3 mitochondrial, putative, mL71, SpoU_methylase domain-containing protein, Ribosomal protein L4/L1 family, putative, Tetratricopeptide repeat, SpoU_methylase domain-containing protein, mt-LAF7, mL74, mt-LAF8, RIBOSOMAL_L9 domain-containing protein, mL75, UNK, mL76, Ribosomal protein L11, putative, Chaperone protein DNAj, putative, UNK, mL78, mt-LAF12, GTP-binding protein, putative, UNK, 50S ribosomal protein L13, putative, mL80, mt-LAF14, mL81, mt-LAF15a, Ribosomal_L18e/L15P domain-containing protein, Peptidyl-prolyl cis-trans isomerase, 50S ribosomal protein L17, putative, mL84, Acyl carrier protein, mL85, Lipase (Class 3), bL19m, mL86, mt-LAF19, bL20m, mL87, bL21m, mL88, uL22m, mL89, uL23m, uL24m, Peptidyl-prolyl cis-trans isomerase, mL93, mL94, mL95, mL41, mL42, mL98, L51_S25_CI-B8 domain-containing protein, mt-LAF27, KRIPP9, mt-LAF29, mL49, UNK, UNK, UNK, mL52, mL53, UNK, UNK, mL63, mL64

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)