EMDB EMD-10898

Single particle reconstruction
2.5Å resolution

Acinetobacter baumannii ribosome-tigecycline complex - 50S subunit

Map released:
Overview of EMD-10898
Fitted atomic model: 6ysi
Related EM entries by publication: EMD-10809, EMD-10869, EMD-10892, EMD-10914, EMD-10915
Primary publication:
Structure of the 70S Ribosome from the Human Pathogen Acinetobacter baumannii in Complex with Clinically Relevant Antibiotics.
Nicholson D, Edwards TA, O'Neill AJ, Ranson NA
Structure - (2020)
PMID: 32857965

Function and Biology Details

Sample name: Acinetobacter baumannii ribosome-tigecycline complex - 50S subunit
Ligands: MAGNESIUM ION, TIGECYCLINE, ZINC ION
Nucleic acids: 23S ribosomal RNA, 5S ribosomal RNA, E-site tRNA
Proteins: Acinetobacter baumannii ribosome-tigecycline complex - 50S subunit, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L31, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36

Experimental Information Details

Resolution: 2.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)