EMDB EMD-10775

Single particle reconstruction
3.1Å resolution

Structure of a human 48S translational initiation complex - 40S body

Map released:
Overview of EMD-10775
Source organism: Human [9606]
Fitted atomic model: 6ybw
Related EM entries by publication: EMD-10769, EMD-10772, EMD-10773, EMD-10774, EMD-11302
Primary publication:
Structure of a human 48Stranslational initiation complex.
Brito Querido J, Sokabe M, Kraatz S, Gordiyenko Y, Skehel JM, Fraser CS, Ramakrishnan V
Science 369 1220-1227 (2020)
PMID: 32883864

Function and Biology Details

Sample name: Human 48S initiation complex
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: 18S rRNA, mRNA
Proteins: Human 48S initiation complex, Human 48S initiation complex, Human 48S initiation complex, Human 48S initiation complex, 40S ribosomal protein S4, X isoform, 40S ribosomal protein S11, 40S ribosomal protein S23, 40S ribosomal protein S9, 40S ribosomal protein S7, 40S ribosomal protein S30, 40S ribosomal protein S27, 40S ribosomal protein S21, 40S ribosomal protein S15a, 40S ribosomal protein S17, 40S ribosomal protein S2, 40S ribosomal protein S3a, 40S ribosomal protein SA, 40S ribosomal protein S26, 40S ribosomal protein S14, 40S ribosomal protein S6, 40S ribosomal protein S8, 40S ribosomal protein S24, Eukaryotic translation initiation factor 1A, X-chromosomal, Eukaryotic translation initiation factor 1, 40S ribosomal protein S13, Eukaryotic translation initiation factor 3 subunit J, Eukaryotic translation initiation factor 3 subunit C, 60S ribosomal protein L41

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)