EMDB EMD-10519

Single particle reconstruction
2.8Å resolution

Structure of an archaeal ABCE1-bound ribosomal post-splitting complex

Map released:
Last modified:
Overview of EMD-10519
Fitted atomic model: 6tmf
Primary publication:
Molecular analysis of the ribosome recycling factor ABCE1 bound to the 30S post-splitting complex.
Nurenberg-Goloub E, Kratzat H, Heinemann H, Heuer A, Kotter P, Berninghausen O, Becker T, Tampe R, Beckmann R
Embo J. e103788-e103788 (2020)
PMID: 32064661

Function and Biology Details

Sample name: 30S ABCE1 post-splitting complex
Ligands: MAGNESIUM ION, ZINC ION, IRON/SULFUR CLUSTER, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
Nucleic acid: 16S ribosomal RNA
Proteins: 30S ABCE1 post-splitting complex, 30S small ribosomal subunit, ABCE1, 30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S3Ae, 30S ribosomal protein S4, 30S ribosomal protein S4e, 30S ribosomal protein S5, 30S ribosomal protein S6e, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S8e, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14 type Z, 30S ribosomal protein S15, 30S ribosomal protein S17, 30S ribosomal protein S17e, 30S ribosomal protein S19P, 30S ribosomal protein S19e, RNA-binding protein, 30S ribosomal protein S24e, 30S ribosomal protein S27e, 30S ribosomal protein S27ae, 30S ribosomal protein S28e, 50S ribosomal protein L7Ae, LSU ribosomal protein L41E, ATPase

Experimental Information Details

Resolution: 2.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)