EMDB EMD-10446

Single particle reconstruction
20.0Å resolution

SAGA-TBP filtered to show the position of the DUB domain

Map released:
Overview of EMD-10446
Fitted atomic model: 6tbm
Related EM entries by publication: EMD-10438, EMD-10440, EMD-10441, EMD-10447, EMD-10448
Primary publication:
Structure of SAGA and mechanism of TBP deposition on gene promoters.
Papai G, Frechard A, Kolesnikova O, Crucifix C, Schultz P, Ben-Shem A
Nature 577 711-716 (2020)
PMID: 31969704

Function and Biology Details

Sample name: SAGA bound to TBP
Ligand: water
Proteins: SAGA bound to TBP, TATA-box binding protein (TBP), SAGA bound to TBP, SAGA bound to TBP, SAGA, TATA-binding protein, Transcriptional coactivator HFI1/ADA1, Subunit of SAGA histone acetyltransferase complex, Spt20, Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to act, Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex, Transcription initiation factor TFIID subunit 10, Subunit (61/68 kDa) of TFIID and SAGA complexes, Subunit (90 kDa) of TFIID and SAGA complexes, Subunit (60 kDa) of TFIID and SAGA complexes, Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation, Transcription-associated protein, Transcriptional regulator involved in glucose repression of Gal4p-regulated genes, Spt8, Polyubiquitin-B, Ubiquitin carboxyl-terminal hydrolase, SAGA-associated factor 11, Transcription and mRNA export factor SUS1

Experimental Information Details

Resolution: 20.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)