EMDB EMD-10406

Single particle reconstruction
3.05Å resolution

OCT4-SOX2-bound nucleosome - SHL-6

Map released:
Last modified:
Overview of EMD-10406
Source organisms:
Fitted atomic model: 6t90
Related EM entry by publication: EMD-10408, EMD-10864
Primary publication:
Mechanisms of OCT4-SOX2 motif readout on nucleosomes.
Michael AK, Grand RS, Isbel L, Cavadini S, Kozicka Z, Kempf G, Bunker RD, Schenk AD, Graff-Meyer A, Pathare GR, Weiss J, Matsumoto S, Burger L, Schubeler D, Thoma NH
Science 368 1460-1465 (2020)
PMID: 32327602

Function and Biology Details

Sample name: Ternary complex of OCT4-SOX2 and SHL-6 nucleosome
Ligand: PENTANEDIAL
Nucleic acids: DNA (146-MER), DNA (146-MER)
Proteins: Ternary complex of OCT4-SOX2 and SHL-6 nucleosome, Histones, DNA, G protein/GFP fusion protein,POU domain, class 5, transcription factor 1 (OCT4), SOX2, Histone H3.1, Histone H4, Histone H2A type 1-B/E, Histone H2B type 1-J, Histone H3.1, Green fluorescent protein,POU domain, class 5, transcription factor 1, Transcription factor SOX-2

Experimental Information Details

Resolution: 3.05Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)