EMDB EMD-10380

Single particle reconstruction
3.11Å resolution

Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 and nascent chain-associated complex

Map released:
Overview of EMD-10380
Source organisms:
Fitted atomic model: 6t59
Primary publication:
TTC5 mediates autoregulation of tubulin via mRNA degradation.
Lin Z, Gasic I, Chandrasekaran V, Peters N, Shao S, Mitchison TJ, Hegde RS
Science - (2019)
PMID: 31727855

Function and Biology Details

Sample name: Rabbit 80S ribosome synthesizing beta-tubulin in complex with TTC5
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: P-site tRNA, 28S ribosomal RNA, 5S ribosomal RNA, 5.8S ribosomal RNA
Proteins: Rabbit 80S ribosome synthesizing beta-tubulin in complex with TTC5, Ribosome, Tetratricopeptide repeat protein 5, Ribosomal protein L8, uL3, uL4, 60S ribosomal protein L5, 60S ribosomal protein L6, Ul30, eL8, uL6, 60S ribosomal protein L10, Ribosomal protein L11, eL13, Ribosomal protein L14, Ribosomal protein L15, uL13, uL22, eL18, eL19, eL20, eL21, eL22, Ribosomal protein L23, eL24, uL23, Ribosomal protein L26, 60S ribosomal protein L27, uL15, eL29, eL30, eL31, eL32, eL33, eL34, uL29, 60S ribosomal protein L36, Ribosomal protein L37, eL38, eL39, eL40, 60s ribosomal protein l41, eL42, eL43, eL28, uL10, Ribosomal protein L12, Nascent polypeptide-associated complex subunit alpha N-terminal region, Nascent polypeptide-associated complex subunit alpha, Transcription factor BTF3, Tetratricopeptide repeat protein 5, Tubulin Beta

Experimental Information Details

Resolution: 3.11Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)