EMDB EMD-10245

Single particle reconstruction
3.3Å resolution

Structure of the RagAB peptide importer in the 'open-closed' state

Map released:
Overview of EMD-10245
Fitted atomic model: 6smq
Related EM entry by publication: EMD-10241, EMD-10243
Primary publication:
Structural and functional insights into oligopeptide acquisition by the RagAB transporter from Porphyromonas gingivalis.
Madej M, White JBR, Nowakowska Z, Rawson S, Scavenius C, Enghild JJ, Bereta GP, Pothula K, Kleinekathoefer U, Basle A, Ranson NA, Potempa J, van den Berg B
Nat Microbiol - (2020)
PMID: 32393857

Function and Biology Details

Sample name: RagAB with putative peptide substrate
Ligands: (1R,4S,6R)-6-({[2-(ACETYLAMINO)-2-DEOXY-ALPHA-D-GLUCOPYRANOSYL]OXY}METHYL)-4-HYDROXY-1-{[(15-METHYLHEXADECANOYL)OXY]METHYL}-4-OXIDO-7-OXO-3,5-DIOXA-8-AZA-4-PHOSPHAHEPTACOS-1-YL 15-METHYLHEXADECANOATE, PALMITIC ACID, (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE
Proteins: RagAB with putative peptide substrate, Lipoprotein RagB, RagA protein, SER-GLY-ALA-THR-THR-ALA-THR-THR-THR-THR-SER-ASN-SER, RagA protein

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)