EMD-10204

Single-particle
4.4 Å
EMD-10204
3D View Gallery
Deposition: 08/08/2019
Map released: 06/11/2019
Last modified: 22/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-10204
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6shs summary report (pdf.gz) Map and 6shs FULL report (pdf.gz)
EMICSS annotations (xml)

Abeta fibril (Morphology I)

EMD-10204

Single-particle
4.4 Å
EMD-10204
3D View Gallery
Deposition: 08/08/2019
Map released: 06/11/2019
Last modified: 22/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Helical Array
Specimen preparation [1]
Buffer
pH: 7.0
Grid
Mesh: 200
Model: C-flat-1.2/1.3
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Time: {'units': 's', 'valueOf_': '40'}
Atmosphere:
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 294 K
Instrument: GATAN CRYOPLUNGE 3
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.0 µm - 3.0 µm
Nominal magnification: 105000.0
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Frames per image: 1-40
Number of real images: 3027
Average exposure time: 24.0 s
Average electron dose per image: 40.9 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.4 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 12282
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: INSILICO MODEL
⦨ Initial angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
RELION - -
⦩ Final angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
RELION - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: ABeta(1-40) Morphology I
⬡ Geometry
X Y Z
Dimensions 150 150 150
Origin 0 0 0
Spacing 150 150 150
Voxel size 1.35 Å 1.35 Å 1.35 Å
Contour list
Primary Level Source
True 0.032 AUTHOR